Yeast two-hybrid system
نویسندگان
چکیده
Introduction The two-hybrid system (THS), first introduced by Fields and Song (1989) (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest. Here, we present not a review of the current THS technology but, rather, a comprehensive guide designed to take the reader through a THS screen. Readers not familiar with the THS are asked to review (Ref. 1, 2, 3, 4, 5). Currently, there are a number of different versions of the THS available. This article describes procedures useful for the `Fields' THS, based on the GAL4 transcription factor. Many of the following protocols can be used with other versions with some modification; however, they might not be directly applicable to the Brent Interaction Trap version because of some distinct differences (Ref. 6).
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